biochemistry
Updated: 2010-02-23 03:47:34
part of biochemistry tutorial 1. LU1. click here to download
The incorporation of thymidine analogues, such as 5-bromo-2′-deoxyuridine (BrdU), into newly synthesized DNA is a powerful tool for analysis of DNA replication, repair and other aspects of DNA metabolism. In Genome-Wide Analysis of DNA Synthesis by BrdU Immunoprecipitation on Tiling Microarrays (BrdU-IP-chip) in Saccharomyces cerevisiae, Oscar Aparicio and colleagues from the University of Southern California [...]
mRNA in situ hybridization is a standard laboratory technique for analyzing gene expression. In a small, transparent specimen like a zebrafish embryo, this technique is straightforward and works well. Cold Spring Harbor Protocols has a set of protocols (here, here and here) describing the method from Cecilia Moens. But what happens when [...]
As you may already be aware, one of the primary reasons the National Cancer Institute is building th
Water has a fundamental role in life processes. Let us begin analysing its properties and roles. The
I like to learn about things that matter. One of the more basic things that matter in my life is fo
Mapping DNase I hypersensitive sites has long been the standard method for identifying genetic regulatory elements such as promoters, enhancers, silencers, insulators, and locus control regions. Sequences that are nucleosome-depleted, presumably to provide access for transcription factors, are selectively digested by DNase I. Traditional low-throughput methods use Southern blots to then identify these hypersensitive [...]
January’s issue of Cold Spring Harbor Protocols wraps up the second volume of our ongoing Emerging Model Organisms series. The idea behind the series is that technical advances have allowed for great expansion in the range of organisms used for research. Each set of articles is meant to introduce the reader to a [...]
Metagenomics, the study of DNA isolated from naturally occurring populations and samples, is rapidly growing. Improvements to cloning and sequencing techniques are allowing researchers to study organism in environmental samples, and new knowledge of species interactions and community dynamics is emerging. The identification of microorganisms in these samples is of vital importance to [...]
Marshall Pope, PhD has been named director of the Carver College of Medicine (CCOM) Proteomics Core Facility. Together with the addition of several new instruments, Dr. Pope will establish a robust and versatile facility for both qualitative and quantitative identification of proteins and their post-translational modifications. The appointment was effective October 31, 2009.
Dr. Pope and [...]
As the cost of sequencing declines, more and more laboratories are extending their research into metagenomics. The Metagenomics RAST Server (MG-RAST) is one of the leading tools for metagenomic analysis, and in January’s issue of Cold Spring Harbor Protocols, Folker Meyer and colleagues from the Argonne National Laboratory present a detailed set of instructions [...]
Poster registrations are now being accepted for both poster sessions being held in conjunction with this year’s Health Sciences Research Week. The Carver College of Medicine, College of Pharmacy, College of Public Health, Iowa City Veterans Affairs Medical Center and Iowa Institute for Clinical and Translational Science will again be participating in the sessions.
Dates and [...]
The Carver College of Medicine, partnering with its departments, has announced the 2010 Distinguished Biomedical Scholars Lecture Series slate of speakers. The series, still in its inaugural year, features top-tier scientists and physicians performing high impact research in a variety of areas. Three investigators have presented seminars since the kick-off lecture in September. These previous [...]
CCOM investigators utilizing human tissue in their research studies now have a new resource at their disposal. The Carver College of Medicine Tissue Procurement Core Facility received Institutional Review Board (IRB) approval in late November and has begun the process of collecting, storing and logging tissue samples for use in current and future research. The [...]
In 2007, The University of Iowa Center on Aging developed a registry of senior research volunteers to aid UI research studies involving participants from older populations. Today, the Seniors Together in Aging Research (STAR) registry includes over 1,400 individuals age 50 and older and continues to recruit participants and facilitate clinical research.
STAR matches research volunteers [...]
The enzyme-linked immunospot (ELISPOT) assay is considered by many to be the gold standard for monitoring cellular immune responses. The method is highly sensitive, quantitative, easy to use and amenable to high throughput screening. Until recently, the ELISPOT assay has been limited to the characterization of only one single effector molecule. [...]
I wanted to point out an excellent thought-provoking article by my Scholarly Kitchen colleague Michael Clarke, titled Why Hasn’t Scientific Publishing Been Disrupted Already? I know many readers here are interested in the future of scientific communication, and Clarke makes some interesting arguments for why journals are likely to persist.