Cold Spring Harbor Protocols is hosting the movie figures that accompany the new lab manual, Live Cell Imaging, Second Edition, edited by Robert Goldman, Jason Swedlow and David Spector, . These movies are freely accessible to all, and worth a look if you’re interested in seeing the state of the art in time lapse [...]4-dimensional live cell imaging has gone from being a rare technique used only by cutting-edge laboratories to a mainstream method in use everywhere. While more and more labs are becoming comfortable with the equipment and protocols needed to collect imaging data, performing detailed analyses is often problematic. The application of computational image processing [...]
Cells recognize one another because of the saccharides attached to cell surfaces. They are prese
Alaskan beetle Upis ceramboides produces a non-protein "antifreeze" molecule. Photo: Kent With the recent progress in understanding epigenetic mechanisms, methods for profiling patterns of DNA modification have become important tools for analysis of gene regulation. DNA methylation, in which cytosine is modified to form 5-methylcytosine, is a well-characterized epigenetic modification essential for normal development in plants and mammals. In the December issue of Cold [...]
Book Details Paperback: 480 pages Publisher: Thieme; 2 Rev Enl edition (December 7, 2004) Language: 
Live cell imaging techniques are driving a revolution in biological research. Instead of viewing dead tissues and cells fixed at a particular stage of activity, scientists can now visualize dynamic changes as they happen, permitting a better understanding of complete processes. The revolution has been fueled by the implementation of genetically encoded fluorescent [...]
Breathing carbon dioxide can trigger panic attacks, but the biological reason for this effect has not been understood. A new study by University of Iowa researchers shows that carbon dioxide increases brain acidity, which in turn activates a brain protein that plays an important role in fear and anxiety behavior.
The study, published in the Nov. [...]
We’re getting toward the end of the second volume of our Emerging Model Organisms series in Cold Spring Harbor Protocols, and November’s issue brings us a look at the Hawaiian Bobtail Squid and the genus Dioscorea, or True Yams.
Euprymna scolopes, the Hawaiian Bobtail Squid (our cover model this month, see below) is a cephalopod that’s [...]
The moment I learned about this new project started by MIT, I could think of only one thing, I want to be part of it. The Massachusetts Institute of Technology, have, started a pharmaceutical innovation program to help drug companies adapt some successful approaches now used in aeronautics, like lean management and information-sharing among rivals.
The [...]The tandem affinity purification (TAP) procedure was pioneered in yeast for the purpose of purifying and characterizing protein complexes and has since been adapted for use in many organisms, including mammalian systems. The TAP procedure involves two sequential affinity purification steps to avoid non-specific protein interactions, a common problem in identifying proteins in complexes. [...]
Intel Corp.’s Digital Health Group has introduced a handheld product to convert printed text to digital text, then read it aloud to the user.
The Intel Reader is designed for persons with dyslexia, other learning disabilities or impaired vision, according to the Santa Clara, Calif.-based vendor. The reader includes a high-resolution camera to point and shoot [...]
Alexander Horswill, PhD, assistant professor of microbiology has been selected by the American Society for Microbiology to receive a Merck Irving S. Sigal Memorial Award. The award is one of only two such awards to be conferred by the society in 2010.
The Merck Irving S. Sigal Memorial Award recognizes excellence in basic research in medical [...]Glutathione-S-transferase (GST) fusion proteins are used in a wide variety of applications in the lab. GST was originally selected as a fusion moiety because it’s not sequestered in inclusion bodies when expressed in bacteria and it can be affinity-purified without denaturation. Purification is fairly straightforward process, and GST fusion proteins are routinely used [...]The National Library of Medicine (NLM) has informed us that Cold Spring Harbor Protocols has been selected to be indexed and included in MEDLINE, and that our articles will now be included and searchable using PubMed. We’re very pleased to share this announcement as it should make it easier for readers to find the [...]
Two researchers in the University of Iowa Roy J. and Lucille A. Carver College of Medicine have been renewed for another five years as investigators of the Howard Hughes Medical Institute (HHMI). Both researchers, Kevin Campbell, Ph.D., and Michael Welsh, M.D., have been HHMI investigators since 1989.
Based in Maryland, the institute was founded in 1953 [...]The introduction of high-throughput laboratory methods has greatly increased the pace of research into the genetics of complex diseases. Instead of focusing only on one or a few coding variants in a small sample of individuals, the ability to accurately and efficiently genotype many individuals and to cover more of the variation within individual [...]